Skip to main content
Figure 2 | BMC Biotechnology

Figure 2

From: A novel expression system of domain I of human beta2 glycoprotein I in Escherichia coli

Figure 2

Optimum DI expression observed at lower levels of IPTG induction. Western blot of expression cultures induced with 1 mM, 0.4 mM, 0.1 mM IPTG and an uninduced control. Both non-reduced periplasmic extracts (P) and supernatant (S) samples were analysed using 15% SDS-PAGE and transferred onto a membrane. The membrane was probed with 6C4C10, a monoclonal murine anti-DI antibody. All cultures were performed in parallel under identical conditions and equal volumes applied to the protein gel. Greatest expression of DI was observed from cultures induced with 0.1 mM IPTG.

Back to article page