Figure 4From: Criteria for effective design, construction, and gene knockdown by shRNA vectorsCorrelation between shRNA knockdown efficacy and scoring for six published algorithms for siRNA. Algorithm scores for each shRNA target site from Table 2 are plotted against observed knockdown efficiency for the Hsieh et al. (A), 5' ΔΔG (free energy differential) (B), Reynolds et al. (C), Amarzguioui et al. (D), Ui-Tei et al. (E) and Takasaki et al. (F) algorithms. The 5' ΔΔG score is plotted on a reverse horizontal axis since knockdown efficacy is predicted to correlate with negative 5' ΔΔG value. A trend line is shown along with the R2 value for each plot. Knockdown of less than 10% is plotted as zero.Back to article page