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Figure 8 | BMC Biotechnology

Figure 8

From: Pore forming polyalkylpyridinium salts from marine sponges versus synthetic lipofection systems: distinct tools for intracellular delivery of cDNA and siRNA

Figure 8

Delivery of β-actin siRNA using lipofectamine knocked down β-actin. A, Western blots of β-actin expression in HEK 293 cells and knockdown by delivery of β-actin siRNA using lipofectamine (Lipofect), but not poly-APS, in the absence of serum in the incubation media for 3 h and 6 h. The presence of 10 % serum has been shown to attenuate poly-APS-mediated pore formation, so experiments were conducted in serum-free conditions. B, shows a standard loading record for NFKB for the Western blots of siRNA knockdown of β-actin by LipoGen, poly-APS and lipofectamine (Lipofect) illustrated in part C of this figure. C, Western blots for siRNA knockdown of β-actin by LipoGen, lipofectamine (Lipofect) and poly-APS.

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BMC Biotechnology

ISSN: 1472-6750

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