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Figure 2 | BMC Biotechnology

Figure 2

From: Pore forming polyalkylpyridinium salts from marine sponges versus synthetic lipofection systems: distinct tools for intracellular delivery of cDNA and siRNA

Figure 2

DNA condensation profile of poly APS and LipoGen using different DNA; pEGFP (4.7 kilobase pairs), pGL3 (5.3 kilobase pairs) and calf thymus DNA (13 kilobase pairs), using ethidium bromide displacement and light scattering assays. Ethidium bromide assay shows a decrease of fluorescence intensity (excitation at 260 nm, and emission at 600 nm) when poly-APS (A) or LipoGen (B) concentration was increased, expressed in a mole ratio of ammonium/DNA phosphate (N/P). Both poly-APS (4.1–4.7 μg/ml) and LipoGen (3.3–5.5 μg /ml) condense DNA efficiently with 10% residual fluorescence at N/P ratios 3.5 – 4.0 and (N/P) 1.5 – 2.5 respectively, (n = 3 for all experiments, error bars represent standard deviation). Light scattering confirmed that cationic vector-DNA complexes were formed, (C) for poly-APS, (D) for LipoGen, as the apparent absorbance at 320 nm increases significantly from the DNA only control solution.

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BMC Biotechnology

ISSN: 1472-6750

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