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Figure 3 | BMC Biotechnology

Figure 3

From: On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg

Figure 3

Far and near UV circular dichroism spectra of fresh and incubated lyophilized and co-lyophilized preparations of subtilisin C. CD spectra are shown for samples freshly suspended in 1,4-dioxane (solid lines) and after further incubation for 4 days (dotted lines)(A) Far UV CD of lyophilized subtilisin C. (B) Near UV CD of lyophilized subtilisin C. (C) Far UV CD of subtilisin C. co-lyophilized with methyl-β-cyclodextrin. (D) Near UV CD of subtilisin C. co-lyophilized with methyl-β-cyclodextrin.

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