Figure 3From: On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin CarlsbergFar and near UV circular dichroism spectra of fresh and incubated lyophilized and co-lyophilized preparations of subtilisin C. CD spectra are shown for samples freshly suspended in 1,4-dioxane (solid lines) and after further incubation for 4 days (dotted lines)(A) Far UV CD of lyophilized subtilisin C. (B) Near UV CD of lyophilized subtilisin C. (C) Far UV CD of subtilisin C. co-lyophilized with methyl-β-cyclodextrin. (D) Near UV CD of subtilisin C. co-lyophilized with methyl-β-cyclodextrin.Back to article page