Figure 2From: On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin CarlsbergInverted second derivative absorbance spectra of subtilisin: in aqueous medium (dashed line) and after suspension in 1,4-dioxane (solid line) at different times of incubation. Lyophilized powder before incubation (A), and after incubation for 4 days (B). Co-lyophilized enzyme before (C), and after incubation for 4 days (D).Back to article page