Figure 3
From: Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals
Activity of bifunctional oligonucleotides with branched 3' tails. The Bcl-x pre-mRNA was incubated for 2 hours in HeLa extracts in the presence of increasing amounts of oligonucleotides. (A) Schematic structure of the oligonucleotides. The presence in the tail of a branch site (BS) or a 5' splice site (5'ss) is indicated. (B) RT-PCR analysis of splicing mixtures were set up in triplicate. The Bcl-xL/Bcl-xS ratio of amplified products was plotted in the graph relative to the concentration of the oligonucleotides. (C) The profile obtained on the Agilent 2100 bioanalyzer is shown for selected mixtures including a control mixture (lane 2) and mixtures containing oligonucleotides M4, M4-25D and M4-25C at 1.5, 3.0, 6.0 and 7.5 nM. The position and size of the Bcl-x amplified products are shown, as well as the size of molecular weight markers.