Skip to main content

Table 2 Expression analysis of proteins.

From: Multiplexed expression and screening for recombinant protein production in mammalian cells

   Fluorescence Intensity (×104)   
   50 ml culture 2 ml culture   
Vector Protein Western blot Dot blot Ex-IMAC AKTA (µg) Analytical SEC (µg)
#1 Mfap3 0 0 0.4 0 -
#2 CLM9 850.6 657.1 298.4 535.5 124.6
#3 Undefined 1228.5 1054.3 1516.6 833.1 526.0
#4 4732429D16Rik 0 0 15.8 0 -
#5 BC025105 19.6 56.3 9.7 0 -
#6 Fcer1a 795.9 456.0 909.6 256.0 97.8
#7 Cd2 900.6 584.5 432.2 483.1 387.2
#8 Tmem25 0 0 5.6 0 -
#9 Tmem25 0 0 6.8 0 -
#10 Tmem25 53.6 122.3 147.5 117.9 -
#11 F11r 0 0 44.4 79.3 -
#12 Ptpns1 0 0 0.2 0 -
#13 Il18rap 65.4 65.3 152.0 70.3 -
#14 Unc5b 419.7 101.9 206.0 297.3 68.6
#15 Fgfrl1 0 0 5.0 64.6 -
#16 AI415330 312.1 58.3 1081.3 191.2 -
#17 Ly9 494.6 425.5 485.9 387.5 57.6
#18 Scn3b 0 0 5.6 0 -
#19 E130306I01Rik 0 0 25.3 0 -
#20 Kit 0 0 4.2 0 -
#21 Lag3 410.7 428.6 371.1 296.8 73.1
#22 Lrrn1 182.6 90.4 117.4 131.3 -
  1. Fluorescent intensity of bands on western blot and dots on dot blots were analysed using ImageQuant software. The Ex-IMAC AKTA column values are calculated by integrating the elution peak from the sephadex column between the IMAC and ion-exchange steps. The Analytical SEC column values are calculated by analysing the purified proteins (see Methods section). There is a cut off during AKTA purification, if the imidazole strip peak detects < 200 ug the fraction is not collected and therefore no protein is purified and there is nothing to analyse on the SEC. All values are normalised to the same input volume of 50 ml for western blot, dot blot and purification analysis. Abbreviations: IMAC (Immobilised Metal Affinity Chromatography), SEC (Size Exclusion Chromatograpy).