Multiplexed expression and screening for recombinant protein production in mammalian cells

BMC Biotechnology

Table 1 Expression vectors used in study

Vector	Gene name	Ensemble ID	C-tag	Mwt (kDa)
#1	Mfap3	ENSMUSP00000020830	rCD4-His10	36.99
#2	CLM9	ENSMUSP00000017453	rCD4-His10	39.97
#3	Undefined	ENSMUSP00000024791	rCD4-His10	33.43
#4	4732429D16Rik	ENSMUSP00000047022	rCD4-His10	41.51
#5	BC025105	ENSMUSP00000051521	rCD4-His10	42.77
#6	Fcer1a	ENSMUSP00000056882	rCD4-His10	44.67
#7	Cd2	ENSMUSP00000029456	rCD4-His10	45.15
#8	Tmem25¹	ENSMUSP00000002100	rCD4-His10	47.03
#9	Tmem25¹	ENSMUSP00000002100	rCD4-His10	47.03
#10	Tmem25²	ENSMUSP00000002100	rCD4-His10	47.03
#11	F11r	ENSMUSP00000041907	rCD4-His10	47.44
#12	Ptpns1	ENSMUSP00000049022	rCD4-His10	62.61
#13	Il18rap	ENSMUSP00000027237	rCD4-His10	63.21
#14	Unc5b	ENSMUSP00000077080	rCD4-His10	63.45
#15	Fgfrl1	ENSMUSP00000013633	rCD4-His10	62.41
#16	AI415330	ENSMUSP00000059913	rCD4-His10	66.44
#17	Ly9	ENSMUSP00000069319	rCD4-His10	72.27
#18	Scn3b	ENSMUSP00000051627	rCD4-His10	46.60
#19	E130306I01Rik	ENSMUSP00000061906	rCD4-His10	65.66
#20	Kit	ENSMUSP00000005815	rCD4-His10	94.62
#21	Lag3	ENSMUSP00000032217	rCD4-His10	69.63
#22	Lrrn1	ENSMUSP00000037096	rCD4-His10	92.52
Control rCD4	CD4	ENSRNOP00000021915	His10	24.20
Control EfnB2	EfnB2	ENSMUSP00000001319	His8	23.29
Control mCD4	CD4	ENSMUSP00000024044	His10	26.30
Control mCD200	CD200	ENSMUSP00000023341	rCD4-His10	46.57

Expression vectors (#1 – #22) encoding the extra-cellular region of mouse receptor genes used for deep well block transient expression study. Tmem25¹ contained a frameshift mutation and Tmem25² contained no mutations relative to the reference sequence. Included in this table are the four control vectors rat CD4 (domains 3 + 4), mouse EfnB2 (extra-cellular region), mouse CD4 (domains 3 + 4) and mouse CD200 (extra-cellular region).

ISSN: 1472-6750