Figure 6
From: Multiplexed expression and screening for recombinant protein production in mammalian cells
Dot blot calibration curves using three standard proteins. Three purified proteins (quantitated by SEC) diluted in mock supernatant and denatured by addition of urea to a final concentration of 5 M were analysed within a range of 0 to 3000 ng per dot by dot blot. R2 were calculated by linear regression analysis (SigmaPlot, SPSS Inc). Error bars represent standard deviations calculated from the data (n = 3).