Comparison of HEK-293E growth kinetics in 24 well blocks culture and 50 ml flask culture. Cells were seeded at 1 × 106 cells/ml in either a total of 50 ml (flask) or 2 ml (24 well blocks). Two separate 24 well blocks were analysed alongside one large scale flask culture in triplicate. Cells were counted using a Neubauer haemocytometer to calculate cell growth and cell viability. Error bars represent standard deviations calculated from the data (n = 3).