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Figure 3 | BMC Biotechnology

Figure 3

From: A time- and dose-dependent STAT1 expression system

Figure 3

Inducible IFNγ-mediated induction of STAT1 target genes in MEF cell lines. Cells were treated with 100 nM rapalog for 24 h or left untreated and subsequently stimulated with IFNγ (100 U/ml) for 4 h or left untreated. Total RNA was used for RT-PCR analysis for GBP2 and IRF1 expression; cyclophilin was used as endogenous control (A). Quantitative RT-PCR was performed for iNOS expression, HPRT was used for normalization; one representative of two independent experiments is shown (B).

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