Target labelling for the detection and profiling of microRNAs expressed in CNS tissue using microarrays

BMC Biotechnology

Table 2 Comparison of miRNA fold changes in a number of brain derived cell lines by Microarray and RT-PCR

Cell line	miRNA	Fold Change by Microarray	Fold Change by qRT-PCR
EOC 13.31	miR-137	-9.9	-38.3
	miR-222	137	4153
EOC 20	miR-137	-1.7	-19.6
	miR-222	113	290
C8-B4	miR-137	-3.5	-10.7
	miR-222	17.9	102
NB41A3	miR-137	1.01	-3.7
	miR-222	ND	13.9
SK-N-FI	miR-137	11.1	11.4
	miR-222	ND	1.3
IMR-32	miR-137	-2.0	1.04
	miR-222	1.7	12.1

The fold change in expression was determined for six brain derived cell lines in comparison with their expression in the cell line N2a.
ND – miRNA was not detected using this methodology

ISSN: 1472-6750