Figure 4

Sensitivity and specificity plot of detection of amplified RNA targets and labelled cDNA targets versus the detection threshold. Sensitivity was determined by – number of true positives/(number of true positives + number of false negatives) for a number of detection thresholds using the Taqman^® results for comparison. Specificity was similarly calculated as – number of true negatives/(number of true negatives + number of false positives). ▲ denotes labelled-aRNA; ■ denotes labelled-cDNA; ---- denotes specificity; — denotes sensitivity.