Table 2 A summary of the oligonucleotides used to generate and sequence confirm the pSANG expression vectors.
From: A simple vector system to improve performance and utilisation of recombinant antibodies
Primer Designation | Primer sequence (5'-3') |
|---|---|
CysBgSaS | 5'-3cggccatggcccaggtgcagctgcaggcggccgcatgcgcacatcatcatcaccatcacaagctttaataag-3' |
CysBgSaA | 5'-3tcgacttattaaagcttgtgatggtgatgatgatgtgcgcatgcggccgcctgcagctgcacctgggccatggccggct-3' |
SerBgSaS | 5'-3cggccatggcccaggtgcagctgcaggcggccgcatccgcacatcatcatcaccatcacaagctttaataag-3' |
SerBgSaA | 5'-3tcgacttattaaagcttgtgatggtgatgatgatgtgcggatgcggccgcctgcagctgcacctgggccatggccggct-3' |
Leadless | 5'-ctagaaataattttgtttaactttaagaaggagatatacc-3' |
leadlesA | 5'-catgggtatatctccttcttaaagttaaacaaaattattt-3' |
PstMBP | 5'-nnnnnnctgcaggcggccgcaaaaatcgaagaaggtaaactggtaatc-3' |
MBPCysHind | 5'-nnnnnnaagcttgtgatggtgatgatgatgtgcacaagtctgcgcgtctttcagggcttcatcgac-3' |
MBPSerHind | 5'-nnnnnnaagcttgtgatggtgatgatgatgtgcagaagtctgcgcgtctttcagggcttcatcgac-3' |
NcoMBP | 5'-gcccagccggccatggcccaggtgcagctgcaggcggccgcaaaaatcgaagaaggtaaactggtaatc-3' |
mut AP sense | 5'-gcaacgtaccacggcaatatcgat-3' |
mut AP antisense | 5'-atcgatattgccgtggtacgttgc-3' |
pstAlkP | 5'-nnnnnnctgcaggcggccgcaaccccggaaatgcctgttctggaaaaccgg-3' |
alkPSerHind | 5'-nnnnnnaagcttgtgatggtgatgatgatgtgcagacggtactttcagccccagagcggctttcat-3' |
H3flagS | 5'-3agctggactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagtaataaa-3' |
H3flagA | 5'-3agcttttattacttgtcatcgtcatccttgtaatcgatgtcatgatctttataatcaccgtcatggtctttgtagtcc-3' |
T7promoter2 | 5'-gatcgagatctcgatcccgcga-3' |
T7terminator | 5'-gctagttattgctcagcg-3' |