Figure 5From: A simple vector system to improve performance and utilisation of recombinant antibodiesEffect of alkaline phosphatase fusion on performance in ELISA. A series of scFvs, as monomeric scFv or bivalent scFv-AP, recognising the same epitope of HER-2 with different affinities were tested at equimolar scFv concentration (1 × 10-7 M) on ELISA wells coated with HER-2. Detection was by time resolved fluorescence using Europium-N1 labelled anti-FLAG M2 antibody.Back to article page