Figure 2

Fluorescent signal scatter in LATE-PCR assays. Panel A: Kinetic plots of accumulated amplification products detected by the molecular beacon probe in replicate samples. Replicate sets of homozygous normal (red lines) and heterozygous (blue lines) samples for the TSD Δ1278 allele (n = 18 each) were amplified using LATE-PCR and monitored in the course of the reaction using a molecular beacon probe against the normal allele. Each reaction contained 1000 genomes equivalent of genomic DNA. Panel B: Statistical analysis of data in Panel A. Solid lines correspond to the average fluorescence values of each replicate set (red: homozygous normal samples; blue: heterozygous samples), error bars correspond to three-standard deviations of the mean which encompasses 99.7.% of all possible samples in each replicate set distribution. LATE-PCR does reduce the overlap between the error bars of each replicate set compared to Figure 1 but still does not permit unambiguous identification of each genotype.