Table 1 List of plasmids used in this study.
From: The cumate gene-switch: a system for regulated expression in mammalian cells
Plasmid name | Configuration | Promoter | Coding sequence | Purpose |
|---|---|---|---|---|
pAdCMV5-CuOg-LacZ | Repressor | CMV5-CuO | LacZ | Reporter for transient transfection |
pAdCMV5-CuOs-LacZ | Repressor | CMV5-CuO | LacZ | Reporter for transient transfection and adenovirus for screening stable transfectants of CymR |
pAdCMV5-CuO-GFP | Repressor | CMV5-CuO | GFP | Reporter for stable expression of repressor configuration |
pAdCMV5-CymR-K7-BFP | Repressor | CMV5 | CymR | Repressor expression in transient transfection assays |
pMPG-BFP/CMV5- CymR/tk-neo | Repressor | CMV5 | CymR | Stable expression of CymR |
pAdCR5-LacZ | Activator | CR5 | LacZ | Reporter gene for transient transfection. |
pAdCMV5-cTA- K7BFP | Activator | CMV5 | cTA | Expression of activator for transient transfection |
pcDNA-cTA | Activator | CMV | cTA | Stable expression of activator |
pAdPS-CMV-cTA-DC- GFP | Activator | CMV | cTA | Transfer vector for generating adenoviral library for mutant cTAs |
pAdCMV5-rcTA- K7BFP | Reverse activator | CMV5 | rcTA | Expression of reverse activator for transient transfection |
pRRL.cppt.CMV5- CuO-rcTA.WPRE | Reverse activator | CMV5-CuO | rcTA | Vector for generating lentivirus expressing the rcTA |
pRRL.cppt.CR5- GFP.WPRE | Activator/Reverse activator | CR5 | GFP | Reporter for lentiviral transduction |
pRRL.cppt.CR5-SEAP-IRES-GFP.WPRE | Activator/Reverse activator | CR5 | SEAP and GFP | Reporter for lentiviral transduction |
pAdCR5-GFP | Activator/repressor | CR5 | GFP | Transfer vector for generating adenovirus for screening cell lines stably expressing cTA. |