Transposition insertion diagram and Southern blot. (A) This diagram represents eight Ap genomic locations and direction of the transposon (arrow under the line) as determined by rescue cloning from GFP/Ap genomic DNA and sequence mapping onto the Ap HZ genome. Four insertions were found in putative coding sequences and four were in intergenic sites. The band sizes on the left of the diagram correspond to bands in the BglII digest lane of the Southern blot. (B) Southern blot of restriction digested GFP/Ap genomic DNA probed with a GFPuv coding sequence probe. The labels indicate restriction enzyme used and the numbers on the right indicate the sizes of visible bands in the BglII lane.