Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A

Table 1 Information on the primers used for real-time PCR

Gene	Primer sequence (5'→3')	Amplicon length	Ta	GenBank accession number or reference
ACTB	TCTGGCACCACACCTTCT TGATCTGGGTCATCTTCTCAC	114 bp	60°C	[GenBank:DQ178122]
B2M	AAACGGAAAGCCAAATTACC ATCCACAGCGTTAGGAGTGA	178 bp	60°C	[GenBank:DQ178123]
GAPDH	ACTCACTCTTCTACCTTTGATGCT TGTTGCTGTAGCCAAATTCA	100 bp	57°C	[GenBank:DQ178124]
HMBS	CTGTTTACCAAGGAGCTGGAAC TGAAGCCAGGAGGAAGCA	100 bp	59°C	[GenBank:DQ178125]
HPRT1	CCGAGGATTTGGAAAAGGT CTATTTCTGTTCAGTGCTTTGATGT	181 bp	60°C	[GenBank:DQ178126]
RPL13A	AGTTAAAGTACCTGGCCTTCCT TGGCCTCTCTTGGTCTTG	136 bp	59°C	[GenBank:DQ178127]
SDHA	GAACCGAAGATGGCAAGA CAGGAGATCCAAGGCAAA	191 bp	58°C	[GenBank:DQ178128]
TBP	GATGGACGTTCGGTTTAGG AGCAGCACAGTACGAGCAA	124 bp	59°C	[GenBank:DQ178129]
TOP2B	AACTGGATGATGCTAATGATGCT TGGAAAAACTCCGTATCTGTCTC	137 bp	60°C	[40]
YWHAZ	ATGCAACCAACACATCCTATC GCATTATTAGCGTGCTGTCTT	178 bp	60°C	[GenBank:DQ178130]
PPARGC1A	CCTGCATGAGTGTGTGCTCT CTCAGAGTCCTGGTTGCACA	107 bp	59°C	[18]

ISSN: 1472-6750