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Table 1 List of recombinant viruses and shuttle and BAC plasmid clones used for their generation

From: Flip-Flop HSV-BAC: bacterial artificial chromosome based system for rapid generation of recombinant herpes simplex virus vectors using two independent site-specific recombinases

BAC-derived recombinant virus

ICP4 expression cassette on the vector

Shuttle vector

Integrated pM24-BAC-shuttle clone

Description

bM24-CMV

pFLS-CMV

pM24-BAC-CMV

The CMV promoter drives the ICP4 expression.

bM24-null

pFLS-ICP4

pM24-BAC-null

No active promoter drives the ICP4 expression.

bM24-empty

NONE

pFLS2

pM24-BAC-empty

No ICP4 CDS included on the vector. (A shuttle vector containing no ICP4 CDS was constructed)

bM24-BAC

N/A

N/A

pM24-BAC (Non-integrated prototype HSV-BAC)

Genetically identical to the prototype HSV-BAC virus M24-BAC

  1. (N/A: not applicable)