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Table 2

From: Development of a new bicistronic retroviral vector with strong IRES activity

Site

Compatible

BamHI

BglII/BclI

PvuII

Any Blunt

EcoRI

MfeI

XhoI

SalI/PspXI

AvaIII

PstI/Sse8387I

ClaI

AsuII/AccI (*)/AclI/AcyI/NarI

AgeI

Cfr9I (SmaI**)/BspMII/BetI/Cfr10I/NgoAIV (NaeI**)/SgrAI

NotI

Bsp120I (ApaI**)/CfrI/XmaIII

SacII

 

SplI

Asp718I (KpnI**)/Bsp1407I/TatI

StuI

Any Blunt

SphI

NspI

BspMII

Cfr9I (SmaI**)/AgeI/BetI/Cfr10I/NgoAIV (NaeI**)/SgrAI

Sse8387I

PstI/AvaIII

AsuII

ClaI/AccI (*)/AclI/AcyI/NarI

SalI

XhoI/PspXI

AccI

ClaI/AsuII/AclI/AcyI/NarI

HinDII

Any Blunt

BglII

BamHI/BclI

MfeI

EcoRI

  1. Polylinker structure.
  2. Sites present in the polylinker, 5' to 3', are indicated in bold type. Compatible sites are shown on the right.
  3. *: only when "MK" from AccI GTMKAC site is CG (K = G or T, M = A or C).
  4. **: prototype site with distinct cleavage structure.