Figure 5
From: Development of a new bicistronic retroviral vector with strong IRES activity
Comparison of stable pPRIG-HA-Red, pPRIG-Hd-HA-Red and pAP2-HA-Red expressions. HEK 293T cells were cotransfected with each vector and helper constructs. Viral supernatants were collected after 2 days and applied to primary rat embryo fibroblasts. The REF cells were photographed two weeks post-transduction. Vis: phase contrast image. Exposure time was the same for the three GFP images, as well as for the three RFP images. Fluorescence intensities are thus a direct reflection of protein expression levels.