Figure 2From: Development of a new bicistronic retroviral vector with strong IRES activityStructure of the four pPRIG vector polylinkers. pPRIG is the prototype polylinker, while the three pPRIG-HA vectors contain additional 1-nucleotide frame-shifted coding sequences for HA-tagging 5' of the polylinker sequence. Any insert can thus be cloned either in-frame downstream of this HA-tag, or directly into the pPRIG, without the HA-tag.Back to article page