Bifunctional recombinant fusion proteins for rapid detection of antibodies to both HIV-1 and HIV-2 in whole blood

BMC Biotechnology

Table 2 Reactivity of various recombinant B6Fab fusion proteins to human serum samples

S.No	SERUM SAMPLE	SOURCE OF SAMPLE	IDENTITY	DILUTION OF SERUM GIVING END POINT OF AGGLUTINATION
				B6Fab	B6Fab	B6Fab	B6Fab	B6Fab	B6Fab	B6Fab	B6Fab	B6Fab
				env1S	env1C	env2S	env2C	env12SS	env12SC	env12CS	env12CC
1.	Cal 21	Calcutta	HIV-1	0	1600	nd	nd	0	nd	nd	nd	0
2.	Cal 22	Calcutta	HIV-1	0	1600	nd	nd	0	nd	nd	nd	0
3.	C-91	Vellore	HIV-1	nd	12800	nd	0	1600	6400	12800	12800	0
4.	C-92	Vellore	HIV-1	nd	12800	nd	0	800	1600	12800	12800	0
5.	C-93	Vellore	HIV-1	nd	51200	nd	0	0	12800	51200	51200	0
6.	C-95	Vellore	HIV-1	nd	>25600	nd	0	>25600	>25600	>25600	>25600	0
7.	PRZ202-07	BBI, USA	HIV-1	nd	25600	nd	0	3200	6400	>25600	25600	0
8.	266/97	Chandigarh	HIV-1+2	12800	>25600	1600	3200	25600	>25600	>25600	>25600	0
9.	288/97	Chandigarh	HIV-1+2	3200	25600	800	3200	6400	6400	>25600	25600	0
10.	C-74	Chandigarh	HIV-1+2	nd	>25600	nd	25600	25600	>25600	>25600	>25600	0
11.	PRZ202-12	BBI, USA	HIV-1+2	nd	1600	nd	25600	6400	25600	12800	25600	0
12.	575/97	Chandigarh	HIV-2	0	0	>25600	>25600	25600	>25600	>25600	>25600	0
13.	PRZ202-01	BBI, USA	HIV-2	nd	0	nd	>25600	>25600	>25600	>25600	>25600	0
14.	PRZ202-03	BBI, USA	HIV-2	nd	0	nd	12800	6400	12800	12800	12800	0
15.	PRZ202-05	BBI, USA	HIV-2	nd	0	nd	>25600	>25600	>25600	>25600	>25600	0
16.	PRZ202-09	BBI, USA	HIV-2	nd	0	nd	6400	3200	6400	6400	6400	0
17.	871	Chandigarh	Negative	0	0	0	0	0	0	0	0	0
18.	872	Chandigarh	Negative	0	0	0	0	0	0	0	0	0
19.	9E10	MAb	Control	25600	25600	12800	25600	12800	>25600	>25600	>25600	25600

Identity of samples is based on data provided by sample supplier as indicated in source of sample. Description of various fusion proteins is given in Fig. 2.
nd-not done

ISSN: 1472-6750