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Table 4 Primers and fluorogenic probes for Real-time PCR

From: Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

Target/amplicon length Specificity/sequence accession number Primer and probe names Primer/probe sequence Lengh (bp) Reference
Lectin
81 bp
soybean specific gene (Accession number K00821) TM-Lectin-F 5'-tcc acc ccc atc cac att t-3' 19 29
   TM-Lectin-R 5'-ggc ata gaa ggt gaa gtt gaa gga-3' 24  
   Lectin-FAM 5'-FAM-aac cgg tag cgt tgc cag ctt cg-TAMRA-3' 23  
Invertase
79 bp
maize specific gene (Accession number U16123) ivr1-TM1 5'-tgg cgg acg acg act tgt-3' 18 30
   ivr1-TM2 5'-aaa gtt tgg agg ctg ccg t-3' 19  
   ivr 5'-VIC-cga gca gac cgc cgt gta ctt cta cc-TAMRA-3' 26  
p35S
82 bp
p35S promoter (Accession number V00141) TM-35S-1 5'-gcc tct gcc gac agt ggt-3' 18 29, 31
   TM-35S-2 5'-aag acg tgg ttg gaa cgt ctt c-3' 22  
   35S 5'-FAM-caa aga tgg acc ccc acc cac g-TAMRA-3' 22  
tNOS
151 bp
tNOS terminator (Accession number V00087) tNOSF 5'-gtc ttg cga tga tta tca tat aat ttc tg-3' 29 32
   tNOSR 5'-cgc tat att ttg ttt tct atc gcg t-3' 25  
    5'-FAM aga tgg gtt ttt atg att aga gtc ccg caa-TAMRA-   
   tNOSpro 3' 30  
GTS 40-3-2 soybean
83 bp
construct specific (Accession number AB209952) RRS-F 5'-gcc atg ttg tta att tgt gcc at-3' 23 29
   RRS-R 5'-gaa gtt cat ttc att tgg aga gga c-3' 25  
    5'-FAM-ctt gaa aga tct gct aga gtc agc ttg tca gcg-   
   RRS TAMRA-3' 33  
MON810 maize
115 bp
event specific (Accession number AF434709) Mon810F1311 5'-cct tca taa cct tcg ccc g-3' 19 33
   Mon810R1311 5'-aat aaa gtg aca gat agc tgg gca-3' 24  
    5'-FAM-acg aag gac tct aac gtt taa cat cct ttg cca-   
   Mon810pro1311 TAMRA-3' 33