SPECIES SPECIFIC GENE
|
TRANSGENE
|
GMO % ESTIMATE
|
---|
E(amplicon)
|
Slope
|
expected Ct
|
Estimation of the initial copy number
|
E(amplicon)
|
Slope
|
expected Ct
|
Estimation of the initial copy number
|
% of GMO
|
% of GMO, varying eff. of species specific gene only
|
---|
1.15
|
3.01
|
19.92
|
367470
|
1.15
|
3.01
|
23.84
|
24384
|
6.64
|
1.18
|
1.1
|
3.10
|
20.55
|
237153
|
1.1
|
3.10
|
24.59
|
14440
|
6.09
|
1.83
|
1.05
|
3.21
|
21.24
|
147004
|
1.05
|
3.21
|
25.42
|
8148
|
5.54
|
2.96
|
1
|
3.32
|
22.00
|
87000
|
1
|
3.32
|
26.32
|
4350
|
5.00
|
5.00
|
0.95
|
3.45
|
22.83
|
48803
|
0.95
|
3.45
|
27.32
|
2178
|
4.46
|
8.91
|
0.9
|
3.59
|
23.76
|
25720
|
0.9
|
3.59
|
28.43
|
1012
|
3.94
|
16.91
|
0.85
|
3.74
|
24.79
|
12596
|
0.85
|
3.74
|
29.66
|
431
|
3.42
|
34.53
|
- * The % of GMO was calculated assuming a PCR efficiency for the transgene amplicon equal to that for the standard curve (= 1.0); only the efficiency of the species specific gene was assumed to change.