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Table 2 PCR efficiency for different soybean and maize matrixes. PCR efficiencies are listed for different soybean and sample matrixes for the species specific gene and the transgene (CV = coefficient of variation [%]).

From: Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

  

SPECIES SPECIFIC GENE

TRANSGENE

 

Sample

slope of the standard curve

PCR efficiency

correlation coefficient (R2)

slope of the standard curve

PCR efficiency

correlation coefficient (R2)

SOYBEAN

CRM

-3.51

0.93

0.9983

-3.46

0.95

0.9926

 

grain

-3.83

0.82

0.9982

-3.56

0.91

0.9528

 

feed

-3.43

0.96

0.9933

-3.15

1.08

0.9520

 

soybean milk

-3.46

0.95

0.9946

-3.69

0.87

0.9664

 

CV

 

7.05

0.25

 

9.56

1.96

MAIZE

CRM

-3.45

0.95

0.9989

-3.23

1.04

0.9878

 

grain

-3.26

1.03

0.9960

-3.10

1.10

0.9570

 

feed

-3.19

1.06

0.9794

-3.23

1.04

0.8736

 

tortilla chips

-3.31

1.01

0.9982

-3.40

0.97

0.9743

 

CV

 

4.59

0.93

 

5.12

5.41