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Table 2 PCR efficiency for different soybean and maize matrixes. PCR efficiencies are listed for different soybean and sample matrixes for the species specific gene and the transgene (CV = coefficient of variation [%]).

From: Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

   SPECIES SPECIFIC GENE TRANSGENE
  Sample slope of the standard curve PCR efficiency correlation coefficient (R2) slope of the standard curve PCR efficiency correlation coefficient (R2)
SOYBEAN CRM -3.51 0.93 0.9983 -3.46 0.95 0.9926
  grain -3.83 0.82 0.9982 -3.56 0.91 0.9528
  feed -3.43 0.96 0.9933 -3.15 1.08 0.9520
  soybean milk -3.46 0.95 0.9946 -3.69 0.87 0.9664
  CV   7.05 0.25   9.56 1.96
MAIZE CRM -3.45 0.95 0.9989 -3.23 1.04 0.9878
  grain -3.26 1.03 0.9960 -3.10 1.10 0.9570
  feed -3.19 1.06 0.9794 -3.23 1.04 0.8736
  tortilla chips -3.31 1.01 0.9982 -3.40 0.97 0.9743
  CV   4.59 0.93   5.12 5.41