intra-assay | inter-assay | CV attributed to final result | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Mean Ct | Copy number estimate | Mean Ct | Copy number estimate | |||||||
Amount of DNA | Mean | SD | CV | Mean | SD | CV | ||||
lectin | 100 ng (87000 copies) | 22.12 | 76459 | 2652.86 | 3.47 | 22.21 | 76665 | 2136.80 | 2.79 | 7.3 |
20 ng (17400 copies) | 24.26 | 19179 | 514.26 | 2.68 | 24.26 | 19561 | 562.87 | 2.88 | 4.4 | |
5 ng (4350 copies) | 26.38 | 4861 | 415.49 | 8.55 | 26.39 | 4705 | 145.13 | 3.08 | 12.5 | |
1 ng (870 copies) | 28.98 | 895 | 32.08 | 3.58 | 28.89 | 884 | 38.35 | 4.34 | 10.4 | |
0.5 ng (435 copies) | 30.25 | 392 | 20.92 | 5.33 | 30.06 | 403 | 13.71 | 3.40 | 10.9 | |
RRS | 100 ng (4350 copies) | 27.99 | 4349 | 87.08 | 2.00 | 28.00 | 4017 | 273.05 | 6.80 | |
20 ng (870 copies) | 30.46 | 827 | 70.50 | 8.52 | 30.34 | 893 | 29.61 | 3.31 | ||
5 ng (218 copies) | 32.39 | 224 | 23.36 | 10.41 | 32.51 | 223 | 27.00 | 12.10 | ||
1 ng (44 copies) | 34.73 | 47 | 8.48 | 18.13 | 34.92 | 49 | 4.69 | 9.49 | ||
0.5 ng (22 copies) | 35.90* | 22 | 5.84 | 27.17 | 36.18* | 22 | 2.30 | 10.34 |