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Figure 6 | BMC Biotechnology

Figure 6

From: Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

Figure 6

Influence of PCR efficiency on GM content determination at different GM concentration ranges. Deviation of the estimated GM content from the true value (in %) is shown when E(sample) deviates form E(standard curve) for 0, 0.05, 0.10, 0.15, 0.20 and 0.25. Overestimation of GM content (in %) when PCR efficiency of the sample E(sample) is higher then the PCR efficiency of the standard curve is shown for 5% (A), 2.5% (C) and 1% GMO (E). Underestimation of GM content (in %) when PCR efficiency of the sample E(sample) is lower then the PCR efficiency of the standard curve is shown for 5% (B), 2.5% (D) and 1% GMO (F). (E = PCR efficiency).

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BMC Biotechnology

ISSN: 1472-6750

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