Adenoviral cytopathic effect assay. Panel A: Monolayers of HT-29, Panc1, and primary human keratinocytes (HKC) were infected with serial dilutions of dl327, and adenoviral vectors carrying the adenoviral E1A region and E4 under the transcriptional control of the COX-2 or Ki-67 promoter, respectively. The indicated MOI (PFU/cell) was determined for each cell line individually. Panel B: monolayers of primary pancreatic carcinoma cells (PPC), HKC, and primary adenoid cells (PAC) were left untreated or infected at an MOI of 5 PFU/cell (determined for each cell line individually) with dl327, Ad.Ki•COX or Ad.COX•Ki, respectively. Five days later, the cells were washed and stained. One representative experiment out of three is shown.