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Figure 2 | BMC Biotechnology

Figure 2

From: Efficient generation of double heterologous promoter controlled oncolytic adenovirus vectors by a single homologous recombination step in Escherichia coli

Figure 2

Luciferase reporter assays. The specificity of the COX-2 and Ki-67 promoter was confirmed using a dual luciferase reporter assay, allowing the analysis of test promoters via firefly luciferase activity and normalization of transfection efficiency via Renilla reniformis luciferase driven by the constitutive CMV-IE promoter in a single-well. Promoter activity was measured in subconfluent or G0/G1-arrested cells. To examine whether adenoviral gene products influence the promoter activity cells were infected with Ad5 after transfection with the reporter plasmids. The activity of the COX-2 and Ki-67 promoter was normalized to that of the CMV-IE promoter. Data are presented as mean ± SD from four independent experiments.

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