Table 3 Inducible luciferase activity in independent clones transformed with pTcINDEX-Luc in pLEW13 or pTcrRNA-T7Tet background.
From: pTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi
Cell line | Uninduced | 0.25 μg ml-1tet | Ratio +tet/-tet |
|---|---|---|---|
CL-LA1N (pLEW13) | 338 (16) | 9831 (411) | 29 |
CL-L1D4 (pLEW13) | 3016 (36) | 75792 (4195) | 25 |
CL-LB5N(pLEW13) | 393 (7) | 7426 (281) | 19 |
CL-L4B1(pLEW13) | 303 (5) | 3196 (256) | 11 |
CL-L4D5(pLEW13) | 848 (37) | 18003 (613) | 21 |
CL-L1B6 (pTcrRNA-T7Tet) | 83249 (2920) | 412729 (33287) | 5 |
CL-L1C4 (pTcrRNA-T7Tet) | 76625 (4521) | 709201 (12746) | 9 |
CL-L2A4 (pTcrRNA-T7Tet) | 91785 (4306) | 425708 (15432) | 5 |
CL-L2D4 (pTcrRNA-T7Tet) | 92374 (7511) | 769333 (44171) | 8 |
CL-L1A6 (pTcrRNA-T7Tet) | 86922 (2736) | 316358 (16381) | 3 |