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Table 3 Inducible luciferase activity in independent clones transformed with pTcINDEX-Luc in pLEW13 or pTcrRNA-T7Tet background.

From: pTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi

Cell line Uninduced 0.25 μg ml-1tet Ratio +tet/-tet
CL-LA1N (pLEW13) 338 (16) 9831 (411) 29
CL-L1D4 (pLEW13) 3016 (36) 75792 (4195) 25
CL-LB5N(pLEW13) 393 (7) 7426 (281) 19
CL-L4B1(pLEW13) 303 (5) 3196 (256) 11
CL-L4D5(pLEW13) 848 (37) 18003 (613) 21
CL-L1B6 (pTcrRNA-T7Tet) 83249 (2920) 412729 (33287) 5
CL-L1C4 (pTcrRNA-T7Tet) 76625 (4521) 709201 (12746) 9
CL-L2A4 (pTcrRNA-T7Tet) 91785 (4306) 425708 (15432) 5
CL-L2D4 (pTcrRNA-T7Tet) 92374 (7511) 769333 (44171) 8
CL-L1A6 (pTcrRNA-T7Tet) 86922 (2736) 316358 (16381) 3
  1. Each clone was induced with 0.25 μg ml-1 tetracycline. After 48 hours, extracts were tested for luciferase activity (Methods). Each extract was assayed in triplicate. Controls were identical cultures maintained in the absence of tetracycline. The activity is represented as mean relative light units per 5 × 104 epimastigotes. Figures in parentheses represent standard error of the mean.