Figure 9
From: pTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi
Immunofluorescence localisation of epitope tagged TcSOD A. Cells were induced as in Fig [8]. The parasites were fixed in paraformaldehyde and stained with mouse monoclonal anti-c-myc 9E10. Slides were examined on a Zeiss LSM 510 confocal microscope. The epitope tagged protein is shown as green fluorescence with the DNA stained red. Arrows indicate the strong staining of a structure adjacent to the kinetoplast (K). The nucleus is indicated (N). The white bar indicates 5 μm. The phase image is shown for comparison. Both images show cells in the process of dividing.