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Figure 5 | BMC Biotechnology

Figure 5

From: pTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi

Figure 5

RFP expression in cloned cells by microscopy. Fluorescence microscopy of cloned pTcINDEX-RFP transformed epimastigotes before and after induction with tetracycline (5 μg ml-1). An aliquot was removed, and fixed every 24 hours for eight days. Cells were examined on a Zeiss LSM 510 microscope. Panel A shows the uninduced population and panel B the same culture 8 days after induction. In each case 1: DNA stained with TOTO-3 (green), 2: RFP expression (red), 3: phase image and 4 is a merged image.

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