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Figure 3 | BMC Biotechnology

Figure 3

From: Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR

Figure 3

Agarose gel separation of PCR products. Lanes M – molecular weight markers (GeneRuler 100 bp DNA Ladder, Fermentas). Lane 1 – PCR positive control using sll1220 sense and antisense primers, and Synechocystis PCC6803 genomic DNA as template. Lane 2 – PCR negative control using sll1220 sense and antisense primers. Lane 3 – PCR using tag1220 for priming and Synechocystis PCC6803 genomic DNA as template. Lane 4 – PCR using tag1220 and sense primer 1220 for priming and Synechocystis PCC6803 genomic DNA as template. Lane 5 – PCR using tag1220 and sense primer 1220 for priming and Synechocystis PCC6803 tagged cDNA as template. Lane 6 – PCR positive control using alr0762 sense and antisense primers, and Anabaena PCC7120 genomic DNA as template. Lane 7 – PCR negative control using alr0762 sense and antisense primers. Lane 8 – PCR using tag0762 for priming and Anabaena PCC7120 genomic DNA as template. Lane 9 – PCR using tag0762 and sense primer 0762 for priming and Anabaena PCC7120 genomic DNA as template. Lane 10 – PCR using tag0762 and sense primer 0762 for priming and Anabaena PCC7120 tagged cDNA as template.

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