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Table 1 Mobilization of gene-trap tTA transposons in the germlines of seed mice.

From: Conditional gene expression in the mouse using a Sleeping Beautygene-trap transposon

Vector Line Chr. (Pos) Copy No. a Seed Mice (T2+/-;SB10+/-) Offspring New Hits b Hits/Gamete Rate/Copy No. c
  4555 ND 3 M 10 0 <1:10 NA
     F 13 0 <1:13  
     M 10 14 1.40  
     M 19 23 1.21  
  4563 ND 50 F 10 6 0.60 0.019
     M (SB10+/+) 10 12 1.20  
     M 11 3 0.27  
T2/GT2/tTA     M 33 24 0.73  
     M 12 10 0.83  
     M 8 4 0.50  
     M 19 10 0.53  
  4583 ND 60 F 8 1 0.13 0.008
     M 17 13 0.76  
     F (pT2 +/+) 8 1 0.13  
     M 10 3 0.30  
  4587 ND 10 M 21 0 <1:21 NA
  6630 ND 4 M 20 1 0.05 0.020
     M 18 2 0.11  
  6632 6 (B3-C1) 5 M 15 20 1.33 0.267
  6657 7 (A3-B2) 10 M 18 8 0.44 0.044
     F 10 10 1.00  
T2/GT3/tTA 6660 11 (B5-C) 27 M 7 26 3.71 0.098
     M 10 18 1.80  
     M 16 43 2.69  
     M 26 79 3.04  
     M 16 59 3.69  
  6682 3 (E1-E3)
10 (C3-D1)
11 M 16 6 0.38 0.063
  6683 9 (F1-F2) 6 M 14 14 1.00 0.091
  1. aTransposon copy number for each transgenic line was determined by Southern blot (see Materials and Methods)
  2. bThe number of single-copy insertions as determined by the presence of new bands on a Southern blot after mobilization by transposase in the germline of seed mice
  3. cReported is the average hits/gemete divided by the copy number