Skip to main content

Table 1 Mobilization of gene-trap tTA transposons in the germlines of seed mice.

From: Conditional gene expression in the mouse using a Sleeping Beautygene-trap transposon

Vector

Line

Chr. (Pos)

Copy No. a

Seed Mice (T2+/-;SB10+/-)

Offspring

New Hits b

Hits/Gamete

Rate/Copy No. c

 

4555

ND

3

M

10

0

<1:10

NA

    

F

13

0

<1:13

 
    

M

10

14

1.40

 
    

M

19

23

1.21

 
 

4563

ND

50

F

10

6

0.60

0.019

    

M (SB10+/+)

10

12

1.20

 
    

M

11

3

0.27

 

T2/GT2/tTA

   

M

33

24

0.73

 
    

M

12

10

0.83

 
    

M

8

4

0.50

 
    

M

19

10

0.53

 
 

4583

ND

60

F

8

1

0.13

0.008

    

M

17

13

0.76

 
    

F (pT2 +/+)

8

1

0.13

 
    

M

10

3

0.30

 
 

4587

ND

10

M

21

0

<1:21

NA

 

6630

ND

4

M

20

1

0.05

0.020

    

M

18

2

0.11

 
 

6632

6 (B3-C1)

5

M

15

20

1.33

0.267

 

6657

7 (A3-B2)

10

M

18

8

0.44

0.044

    

F

10

10

1.00

 

T2/GT3/tTA

6660

11 (B5-C)

27

M

7

26

3.71

0.098

    

M

10

18

1.80

 
    

M

16

43

2.69

 
    

M

26

79

3.04

 
    

M

16

59

3.69

 
 

6682

3 (E1-E3)

10 (C3-D1)

11

M

16

6

0.38

0.063

 

6683

9 (F1-F2)

6

M

14

14

1.00

0.091

  1. aTransposon copy number for each transgenic line was determined by Southern blot (see Materials and Methods)
  2. bThe number of single-copy insertions as determined by the presence of new bands on a Southern blot after mobilization by transposase in the germline of seed mice
  3. cReported is the average hits/gemete divided by the copy number
Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us