Phenotypic rescue by transposon mobilization A). Breeding scheme to remobilize a transposon insertion, 01-0032, out of the mouse Slc25a22 gene as previously reported by Carlson, et al . (2003). Animals for both the single-copy transposon insertion 01-0032 and the CAGGS-SB10 transposase were intercrossed. B) Germline single-copy remobilization rates of the independently generated insertions 01-0032 and 02A-0016 (Additional file 2) were detected by analyzing the donor site for evidence of a transposon footprint by sequencing (data not shown).