Figure 5

Purified virions are immunogenic. Staining with Comassie Brilliant Blue (A) of mock-inoculated control plants (A1), partially purified viral particles of the PVX6HisE2¹2ACP (A2) and PVX6His2ACP (A3). Western blotting with anti-6His (B upper) and anti-CP (B lower) antibodies of partially purified viral particles of the PVX6HisE2¹2ACP (B1) and PVX6His2ACP (B2). Western blotting analysis (C) using sera from rabbit inoculated with PVX6His2ACP against proteins purified from mock-inoculated control plants (C1) and from PVX6His2ACP (C2). Western blotting analysis (D) using sera from rabbit inoculated with PVX6HisE2¹2ACP. Purified E2 protein (D1; E2 control protein is indicated with a bold arrow), marker (D2), proteins purified from plants inoculated with PVX6HisE2¹2ACP (D3), mock-inoculated control plants (D4) and PVX6His2ACP (D5). Standards Molecular weight (kDa = kiloDaltons) are shown on the left of each figure.