PCR-based generation of shRNA libraries from cDNAs

BMC Biotechnology

Table 1 CCND1-YIU library.

No.	Position	Restriction Site	Sequence	Size (bp)	%GC	Orient-ation
1	3727–3746	AluI	CTCACAGTGCTGTGTGCCCC	20	65	+
2	3497–3516	AluI	CTATGGAAGTTGCATAATTA	20	30	+
3	2455–2473	HaeIII	GCAGAGGATGTTCATAAGG	19	47	-
4	1692–1711	HaeIII	CCACGCTACGCTACTGTAAC	20	55	-
5	1934–1952	HaeIII	CTGGTTTTCTACCCAACGG	19	53	+
6	846–864	DpnI	TCAAGTGTGACCCAGACTG	19	53	+
7	765–783	HaeIII	CCGCAGTGCAAGGCCTGAA	19	58	+
8	594–613	HaeIII	CCGCAATGACCCCGCACGAT	20	60	+
9	3318–3336	DpnI	TCCCACACAGGCTGGCGGG	19	74	+
10	312–331	DpnI	TCGTCGCCACCTGGATGCTG	20	65	+
11	1057–1075	HaeIII	CCGGCTCCGCCCTCGCTGC	19	84	-
12	3424–3442	DpnI	TCAGATGAAGTGCCCAGCA	19	53	-
13	1804–1822	HaeIII	GTAGCAGGGTCGGGAAAGG	19	63	+
14	2970–2988	AluI	CTCCATTTTCTTATTGCGC	19	42	+
15	1989–2007	AluI	CTACTTGGTTTGTGTTCTT	19	37	+
16	2752–2771	EcoRI	AGGGGCAGGGGATAAGAATT	20	50	-
17	359–377	HaeIII	CCAGCGGGAAGACCTCCTC	19	68	-
18	1117–1135	AluI	TTGATACCAGAAGGGAAAG	19	42	-
19	Recombined		LacZ
20	Long CCND1			62

CCND1 sense-strand sequences are shown. Nucleotide numbers correspond to [Genbank:BC023620]. Orientation is denoted "+" or "-" if the sequence shown occurs before or after the shRNA loop, respectively.

ISSN: 1472-6750