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Table 2 Fluorescence intensities of soluble protein extracts prepared from heads of transgenic lines expressing reef coral fluorescent proteins.

From: Insulated piggyBac vectors for insect transgenesis

Line Markera Insulatedb Mean Fluorescence/μg proteinc SD
A1 GMR-3xP3-AmCyan Yes 857 173
A2 GMR-3xP3-AmCyan Yes 629 69
A3 GMR-3xP3-AmCyan Yes 846 88
A4 GMR-3xP3-AmCyan Yes 1065 257
A5 GMR-3xP3-AmCyan Yes 1016 83
y w d None   13 37
A7 GMR-3xP3-ZsGreen Yes 758 71
A8M1 GMR-3xP3-ZsGreen Yes 828 41
A8M3 GMR-3xP3-ZsGreen Yes 1558 20
A9 GMR-3xP3-ZsGreen Yes 1205 176
A10 GMR-3xP3-ZsGreen Yes 1213 185
A11 GMR-3xP3-ZsGreen Yes 1737 82
y w None   41 43
R31 3xP3-DsRed1 No 118 24
R30A 3xP3-DsRed1 No 859 56
R19 3xP3-DsRed1 No 215 135
R18 3xP3-DsRed1 No 169 51
R12 3xP3-DsRed1 No 171 31
R21 3xP3-DsRed1 No 342 14
y w None   122 43
  1. aAll lines heterozygous for marker gene
  2. bscs and scs' insulators bracket the GMR-3xP3 marker genes.
  3. cAverage of three protein extracts each prepared from ten homogenized heads from each line. Fluorescence in arbitrary units.
  4. dParental stock used in all germ-line transformation experiments