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Table 2 Fluorescence intensities of soluble protein extracts prepared from heads of transgenic lines expressing reef coral fluorescent proteins.

From: Insulated piggyBac vectors for insect transgenesis

Line

Markera

Insulatedb

Mean Fluorescence/μg proteinc

SD

A1

GMR-3xP3-AmCyan

Yes

857

173

A2

GMR-3xP3-AmCyan

Yes

629

69

A3

GMR-3xP3-AmCyan

Yes

846

88

A4

GMR-3xP3-AmCyan

Yes

1065

257

A5

GMR-3xP3-AmCyan

Yes

1016

83

y w d

None

 

13

37

A7

GMR-3xP3-ZsGreen

Yes

758

71

A8M1

GMR-3xP3-ZsGreen

Yes

828

41

A8M3

GMR-3xP3-ZsGreen

Yes

1558

20

A9

GMR-3xP3-ZsGreen

Yes

1205

176

A10

GMR-3xP3-ZsGreen

Yes

1213

185

A11

GMR-3xP3-ZsGreen

Yes

1737

82

y w

None

 

41

43

R31

3xP3-DsRed1

No

118

24

R30A

3xP3-DsRed1

No

859

56

R19

3xP3-DsRed1

No

215

135

R18

3xP3-DsRed1

No

169

51

R12

3xP3-DsRed1

No

171

31

R21

3xP3-DsRed1

No

342

14

y w

None

 

122

43

  1. aAll lines heterozygous for marker gene
  2. bscs and scs' insulators bracket the GMR-3xP3 marker genes.
  3. cAverage of three protein extracts each prepared from ten homogenized heads from each line. Fluorescence in arbitrary units.
  4. dParental stock used in all germ-line transformation experiments