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Table 1 Fluorescence intensity in the eyes of transgenic lines expressing reef coral fluorescent marker proteins. Live anaesthetized flies were observed with an Olympus SZX12-RFL3 microscope, image captured with an Olympus DP70 digital camera and color intensity mesured using the analySIS software package. Brief exposure times were used to avoid pixel saturation.

From: Insulated piggyBac vectors for insect transgenesis

Line

Markera

Insulatedb

Meanc

SD

A1

GMR-3xP3-AmCyan

Yes

123.9

13.9

A2

GMR-3xP3-AmCyan

Yes

125.3

9.4

A3

GMR-3xP3-AmCyan

Yes

128.6

11.8

A4

GMR-3xP3-AmCyan

Yes

137.6

9.6

A5

GMR-3xP3-AmCyan

Yes

141.3

16.0

y w d

None

 

23.5

2.4

A7

GMR-3xP3-ZsGreen

Yes

75.8

9.5

A8M1

GMR-3xP3-ZsGreen

Yes

82.0

7.8

A8M3

GMR-3xP3-ZsGreen

Yes

93.3

3.2

A9

GMR-3xP3-ZsGreen

Yes

79.5

6.2

A10

GMR-3xP3-ZsGreen

Yes

100.8

4.8

A11

GMR-3xP3-ZsGreen

Yes

101.2

7.1

y w

None

 

17.6

5.2

R31

3xP3-DsRed1

No

20.6

2.4

R30A

3xP3-DsRed1

No

246.7

4.4

R19

3xP3-DsRed1

No

37.5

5.2

R18

3xP3-DsRed1

No

29.9

7.0

R12

3xP3-DsRed1

No

38.5

6.2

R21

3xP3-DsRed1

No

115.6

4.7

y w

None

 

10.5

0.4

  1. aAll lines heterozygous for marker gene.
  2. bscs and scs' insulators bracket the GMR-3xP3 marker genes.
  3. cAverage of three individuals in arbitrary units
  4. dParental stock used in all germ-line transformation experiments