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Table 1 Fluorescence intensity in the eyes of transgenic lines expressing reef coral fluorescent marker proteins. Live anaesthetized flies were observed with an Olympus SZX12-RFL3 microscope, image captured with an Olympus DP70 digital camera and color intensity mesured using the analySIS software package. Brief exposure times were used to avoid pixel saturation.

From: Insulated piggyBac vectors for insect transgenesis

Line Markera Insulatedb Meanc SD
A1 GMR-3xP3-AmCyan Yes 123.9 13.9
A2 GMR-3xP3-AmCyan Yes 125.3 9.4
A3 GMR-3xP3-AmCyan Yes 128.6 11.8
A4 GMR-3xP3-AmCyan Yes 137.6 9.6
A5 GMR-3xP3-AmCyan Yes 141.3 16.0
y w d None   23.5 2.4
A7 GMR-3xP3-ZsGreen Yes 75.8 9.5
A8M1 GMR-3xP3-ZsGreen Yes 82.0 7.8
A8M3 GMR-3xP3-ZsGreen Yes 93.3 3.2
A9 GMR-3xP3-ZsGreen Yes 79.5 6.2
A10 GMR-3xP3-ZsGreen Yes 100.8 4.8
A11 GMR-3xP3-ZsGreen Yes 101.2 7.1
y w None   17.6 5.2
R31 3xP3-DsRed1 No 20.6 2.4
R30A 3xP3-DsRed1 No 246.7 4.4
R19 3xP3-DsRed1 No 37.5 5.2
R18 3xP3-DsRed1 No 29.9 7.0
R12 3xP3-DsRed1 No 38.5 6.2
R21 3xP3-DsRed1 No 115.6 4.7
y w None   10.5 0.4
  1. aAll lines heterozygous for marker gene.
  2. bscs and scs' insulators bracket the GMR-3xP3 marker genes.
  3. cAverage of three individuals in arbitrary units
  4. dParental stock used in all germ-line transformation experiments
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BMC Biotechnology

ISSN: 1472-6750

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