p53-mediated transcriptional transactivation. A : p53-mediated transcriptional transactivation as determined by luciferase activity in SaOs-2 cells co-transfected with a p53-responsive promoter luciferase plasmid and a renilia luciferase expression plasmid for transfection efficiency. Cells were then transduced with the indicated p53 adenoviral vectors. Cell extracts from each triplicate were assayed for both firefly and Renilla luciferases. Results are expressed as the ratio of activity of the firefly to the Renilla luciferase. The average mean +/- SD values and the induction factor relative to the transfection-containing no viral vector from two sets of experiments with triplicate transfections are shown. B: Relative levels of p21waf1/CIP1 protein were measured by immunoblot assay using lysates (80 μg) prepared from SaOs-2 expressing luciferase (mock), p53wt, or p53HRCaax cultured for 48 hr in the presence (+) or absence (-) of the FTI (15 μM). C : Relative levels of Bax protein were measured by immunoblot assay using lysates (80 μg) prepared from SaOs-2 expressing luciferase (mock), p53wt, or p53HRCaax and cultured for 48 hrs in the presence (+) or absence (-) of the FTI (15 μM). One representative of three different experiments is shown.