Subcellular localization of p53 in the different transduced and FTI-treated SaOs-2 cells. A: AdLuc, Adp53wt, Adp53HRSaax, or Adp53HRCaax transduced SaOs-2 cells were untreated or treated with 15 μM FTI for 24 hr. They were then stained with the FITC-conjugated anti human p53 antibody (1:100) and DAPI = 4',6'-diamino-2-phenylindole. The subcellular localization of p53 was examined microscopically. B: Confocal microscopy of transduced Adp53wt or Adp53HRCaax cells treated with FTI for 24 hr.