Figure 1
From: Reversible inactivation of the transcriptional function of P53 protein by farnesylation
Expression of ectopic p53 and endogenous HDJ-2 in human SaOs-2 cells. Cells were transduced with adenoviral vectors expressing the indicated p53 proteins or luciferase as a control. Total protein extracts (80 μg) of SaOs-2 cultures transduced with different Ad-vectors and untreated (-) or treated (+) with 15 μM FTI were prepared 48 hr post transduction and analysed by immunoblotting employing the anti-p53 (1:500) and anti HDJ-2 (1:1000) antibodies.