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Table 2 ESI-MS analysis of the tryptic peptides derived from the fused-protein

From: Construction, purification, and characterization of a chimeric TH1 antagonist

# Peptide sequencea) m/z exp. m/z theor. Zb) Abs. Errorc)
1 11T-K33d) 908.81 908.83 3 0.02
2 40M-K44 639.34 639.35 1 0.01
3 45F-K50 685.33 685.33 1 0.00
4 56A-R66f) 442.87 442.88 3 0.01
5 67A-K114 1781.52 1781.54 3 0.02
6 101Q-K114d), e) 807.93 807.95 2 0.02
7 120N-R151f) 921.14 921.17 4 0.03
8 166S-R173 506.23 506.23 2 0.00
9 177I-K181 511.31 511.28 1 0.03
10 190Q-R220f) 1225.20 1225.23 3 0.03
11 221V-R227d) 456.74 456.75 2 0.01
12 228M-K236d) 535.24 535.25 2 0.01
  1. a) The numbering in the sequences of peptides is according to the fused-protein sequence shown in the section Results and discussion.
  2. b) Charge state of individual peptides.
  3. c) Indicate the absolute mass difference between the theoretical and experimental molecular masses of the detected peptides.
  4. d) Peptides sequenced by ESI-MS/MS.
  5. e) Peptide originated by the non-specific cleavage of trypsin
  6. f) Peptide containing free cysteine.