Figure 2

E coli expression and urea extraction of recombinant chimeric protein. Panel A: Coomasie staining of 12.5% SDS-PAGE in reduced conditions of samples from E coli expression. Lanes A, B, C: E coli strain negative control (empty vector), 0, 8 and 18 hours of induction; Lanes D, E, F: E. coli strains containing the expression plasmid pHu (AnTH1) under induction conditions: 0, 8, and 18 hours of induction. Panel B: Urea extraction. Coomasie staining of 12.5% SDS-PAGE under reducing conditions. Lanes A, B, C, and D, pellets and lanes F, G, H, I, supernatants from extraction with 0, 2, 4 and 8 mol/L urea. Panel C: Lanes A, B, C, and D, pellets and lanes F, G, H, I, supernatants from extraction with 0, 5, 6 and 7 mol/L urea. Lanes E in panels B and C indicate the position of MW. The samples contain approximately the same amount of protein. The arrows indicate the position of AnTh1 recombinant protein