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Table 3 Information on the primers used in this study

From: Selection of a set of reliable reference genes for quantitative real-time PCR in normal equine skin and in equine sarcoids

Gene Genbank accession number Primer sequence Ta (°C) Product size (bp) Primer concentration
ACTB AF035774 CCAGCACGATGAAGATCAAG GTGGACAATGAGGCCAGAAT 59 88 0.5 μl 10 μM
B2M X69083 GTTCCATCCGCCTGGAGATT GGGGGTCTTTGAGAGTAGAGTG 60 182 0.25 μl 10 μM
HPRT1 AY372182 GGCAAAACAATGCAAACCTT CAAGGGCATATCCTACGACAA 60 163 0.5 μl 10 μM
UBB AF506969 GCAAGACCATCACCCTGGA CTAACAGCCACCCCTGAGAC 61 206 0.5 μl 10 μM
TUBA1 AW260995 GCCCTACAACTCCATCCTGA ATGGCTTCATTGTCCACCA 60 78 0.5 μl 10 μM
RPL32 CX594263 AGCCATCTACTCGGCGTCA TCCAATGCCTCTGGGTTTC 61 149 0.25 μl 10 μM
GAPDH AF157626 GATGCCCCAATGTTTGTGA AAGCAGGGATGATGTTCTGG 61 250 0.5 μl 10 μM