Selection of a set of reliable reference genes for quantitative real-time PCR in normal equine skin and in equine sarcoids

Table 3 Information on the primers used in this study

Gene	Genbank accession number	Primer sequence	Ta (°C)	Product size (bp)	Primer concentration
ACTB	AF035774	CCAGCACGATGAAGATCAAG GTGGACAATGAGGCCAGAAT	59	88	0.5 μl 10 μM
B2M	X69083	GTTCCATCCGCCTGGAGATT GGGGGTCTTTGAGAGTAGAGTG	60	182	0.25 μl 10 μM
HPRT1	AY372182	GGCAAAACAATGCAAACCTT CAAGGGCATATCCTACGACAA	60	163	0.5 μl 10 μM
UBB	AF506969	GCAAGACCATCACCCTGGA CTAACAGCCACCCCTGAGAC	61	206	0.5 μl 10 μM
TUBA1	AW260995	GCCCTACAACTCCATCCTGA ATGGCTTCATTGTCCACCA	60	78	0.5 μl 10 μM
RPL32	CX594263	AGCCATCTACTCGGCGTCA TCCAATGCCTCTGGGTTTC	61	149	0.25 μl 10 μM
GAPDH	AF157626	GATGCCCCAATGTTTGTGA AAGCAGGGATGATGTTCTGG	61	250	0.5 μl 10 μM

ISSN: 1472-6750