Growth and production curves for resveratrol biosynthesis. Culture of E. coli pAC-4CL1 + pUC-STS was supplemented with 1 mM 4-coumaric acid and grown for approximately 48 hours in modified M9 medium with glycerol at 30°C. Samples were removed periodically for extraction to determine the consumption of the substrate 4-coumaric acid (○) and the formation of the product resveratrol (●). Growth (■) was monitored spectrophotometrically by measuring OD at 600 nm. Data points represent the mean of three independent measurements.