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Figure 2 | BMC Biotechnology

Figure 2

From: Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli

Figure 2

Expression of Arachis hypogaea STS in E. coli. SDS-PAGE gel (12%) showing the overexpressed STS from peanut roots. The sts gene was cloned into the plasmid pUCMod as described in Materials and Methods. E. coli expressing STS, and a control culture with empty plasmid, were grown overnight in modified M9 medium at 30°C containing glycerol (lanes 1–4) or glucose (lanes 5–7). Cell lysates were prepared as described in Material and Methods. Samples are as follows: Lane 1: empty pUC control, Lane 2: pUC-STS grown in glycerol crude cell lysate, Lane 3: pUC-STS grown in glycerol soluble protein fraction, Lane 4: pUC-STS grown in glycerol insoluble protein fraction, Lane 5: pUC-STS grown in glucose crude cell lysate, Lane 6: pUC-STS grown in glucose soluble protein fraction and Lane 7: pUC-STS grown in glucose insoluble protein fraction, Lane 8: molecular weight standard.

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